Online Agenda

* All times shown in EST

8:30am | Virtual Coffee Morning

9:15am | Chair’s Opening Remarks

Engineering Exosomes for More Efficient Therapies

9:25 am Selecting Preferred Cells for Engineering Exosomes

Synopsis

  • Different cells produce exosomes with different microRNA signatures
  • Can the microRNA profile inform clinical use?
  • Enhancing angiogenic potential by microRNA knockdown

9:50 am Engineering Extracellular Vesicles for the Delivery of Gene Editing Tools

Synopsis

  • Strategies for loading of therapeutic cargo into EVs, and characterization of engineered EV at the single-vesicle and single-molecule level
  • Application of EVs loaded with CRISPR/Cas9 to promote gene editing in in vitro models
  • Safety and in vivo biodistribution of engineered EVs

10:15 am Live Presenter Q&A

Synopsis

  • Ask the speakers your most burning questions!

10:30 am Improvements in Fluorescent Nanoparticle Tracking Analysis: Reliable Characterization of Extracellular Vesicles

Synopsis

• Nanoparticle Tracking Analysis (NTA) has emerged to a vital and fast
characterization technology for exosomes, microvesicles or viruses
• In combination with fluorescence detection (F-NTA), NTA enables the
user to perform biomarker detection on the single particle level, thus
enhancing real EV concentration measurements
• Here we report the multi-fluorescence detection of four independent
biomarkers (CD63, CD81, CD9 and CD41) in one NTA sample for the first
time

11:00am | Virtual Speed Networking

11:45 am PANEL DISCUSSION: Comparing Engineered & Naturally Derived Exosomes for Therapeutic Benefit

Synopsis

  • Is engineering exosomes even necessary? Comparing across disease indications
  • Strategies to test the capacity of both
  • What are the advantages of engineering exosomes as therapeutics and as drug delivery vehicles?
  • Linking engineering to the successful loading of cargo
  • Regulatory considerations of engineering exosomes at scale

Determining the Mechanisms of Action of Exosome-Based Therapeutics

12:30 pm Mechanistic Insights into Extracellular Vesicle-Mediated RNA Delivery

  • Pieter Vader Associate Professor, University Medical Center Utrecht

Synopsis

  • A novel CRISPR-Cas9-based reporter system allows study of EV-mediated functional RNA transfer on a single cell level
  • Regulatory mechanisms in EV-mediated RNA transfer are being explored
  • EVs outperform synthetic carriers in terms of delivery efficiency

12:55 pm Neural Exosome for Delivery to Central Nervous System

  • Steve Stice CSO & Professor, Aruna Bio & University of Georgia

Synopsis

  • Nucleic acid payload delivery and efficacy is enhanced in extracellular vesicles when combinatory approaches are used
  • Neural exosome from non-transformed neural stem cells have specific anti-inflammatory mechanisms of action
  • Neural exosomes are being mined for blood brain barrier and neural specific targets

1:20 pm Live Presenter Q&A

  • Pieter Vader Associate Professor, University Medical Center Utrecht
  • Steve Stice CSO & Professor, Aruna Bio & University of Georgia
  • Joël de Beer Managing Director, Anjarium Biosciences

Synopsis

  • Ask the speakers your most burning questions!

1:35pm | Networking Lunch

2:20 pm Hypothalamic Neural Stem Cell-Based EV Therapeutics

  • Dongsheng Cai Professor, Department of Molecular Pharmacology, Albert Einstein College of Medicine

Synopsis

  • Hypothalamic neural stem cell (htNSC)-secreted EVs: roles for the brain and whole body homeostasis;
  • EV from htNSC subtypes: peptide profile and EV characterization
  • Secretome-based htNSC EV therapeutics for aging and diabetes

2:45 pm Cell and Tissue Reprogramming Using Extracellular Vesicles: Applications in Regenerative Medicine

  • David Greening Associate Professor, Baker Heart & Diabetes Institute

Synopsis

 

    • Quantitative mass spectrometry in defining and understanding extracellular vesicles
    • Targeting extracellular vesicles for delivery – understanding the surfaceome
    • Phosphoproteomic characterization of the EV-mediated signalling network
    • Regenerative approach for cell (free) therapy – strategies for reprogramming cell and tissue environment

3:05 pm Understanding the Roles of Extracellular Vesicles in Rheumatoid Arthritis

Synopsis

  • Details to be revealed

3:35 pm Live Presenter Q&A

Synopsis

  • Ask the speakers your most burning questions!

3:50pm | Afternoon Networking Break

Driving Forward Preclinical Outcomes to Advance into the Clinic

4:30 pm Exosomes Displaying CD24 (EXO-CD24) – A Breakthrough Platform for Amelioration Of Immune Hyper-Activation

  • Shiran Shapira Head of Research Laboratory, Health Promotion Center & Integrated Cancer Prevention Center

Synopsis

  • Development of a new anti inflammatory drug EXO CD24, a combination of immune-checkpoint-protein CD24 with exosomes-based delivery
  • EXO-CD24 was produced by over-expression of CD24 in cells, followed by purification of exosomes from the growth medium
  • EXO-HSA were examined in mouse inflammatory disease models
  • It is suggested that EXO-CD24 may serve as a novel drug platform for pulmonary and systemic diseases with an underlying immune hyperactivation pathology

4:55 pm Preclinical Research of Aegle’s Isolated EVs Promotes Cell Proliferation & Protein Production

Synopsis

• EVs carry the signals of cells and have the potential to be used as a
therapeutic
• EVs induce cell proliferation and angiogenesis in preclinical research
• EVs induce protein production in diseased cells

5:20 pm Bridging the Gap Between Chemotherapy and Immunotherapy; EV Therapeutics Show Promise in Oncology Preclinical Studies

Synopsis

  • Six years of mechanistic and pre-clinical studies will be presented for a novel, first-in-class immunomodulator.
  • Learn how the immune response prevents checkpoint inhibitors from being effective in solid tumors
  • Understand how modified tumor cell -derived extracellular vesicles overcome T-cell exhaustion and synergize with checkpoint inhibitors to potentially allow patient to transition from chemotherapy to immunotherapy

5:45 pm Live Presenter Q&A

Synopsis

  • Ask the speakers your most burning questions!

6:00 pm Chair’s Closing Remarks & End of Conference Day One